Recombinant Human Interleukin-33 (rHuIL-33)

英文全名

Recombinant Human Interleukin-33 (rHuIL-33)

英文别名

IL-1F11, NF-HEV, DVS 27

蛋白结构

Squence of Recombinant Human Interleukin-33

氨基酸序列

SITGISPITE YLASLSTYND QSITFALEDE SYEIYVEDLK KDEKKDKVLL SYYESQHPSN ESGDGVDGKM LMVTLSPTKD FWLHANNKEH SVELHKCEKP LPDQAFFVLH NMHSNCVSFE CKTDPGVFIG VKDNHLALIK VDSSENLCTE NILFKLSET

Accession

O95760

Gene ID

90865

产品英文概述

IL-33, encoded by IL-33 gene located on the Chr.9 in humans, is a 30 kDa proinflammatory protein belonging to the IL-1 superfamily and it shares less than 20 % a.a. sequence identity with other members. IL-33 secreted by high endothelial venules at high levels, which is found in tonsils, peyer patches and mesenteric lymph nodes, but not in placenta. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL1alpha or IL1β stimulation. It elicits its biological effects by interacting with IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. IL-33 mature protein has 52-58 % a.a. sequence identity with mouse and rat IL-33.

来源

Escherichia coli.

分子量

Approximately 17.9 kDa, a single non-glycosylated polypeptide chain containing 159 amino acids.

生物活性

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 107 IU/mg.

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2um filtered concentrated solution in 20 mM PB, 150 mM NaCl, 1mM EDTA, pH7.4.

内毒素

Less than 1 EU/ug of rHuIL-33 as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

纯度

> 97 % by SDS-PAGE and HPLC analyses.
SDS-PAGE of Recombinant Human Interleukin-33

包装规格

10ug/100ug/500ug

安全数据表下载 (SDS)

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技术参数表下载 (TDS)

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品牌链接

Recombinant Human Interleukin-33是ChemWhat品牌授权产品并通过沃尔森销售,下面是ChemWhat上的该产品链接Recombinant Human Interleukin-33 (rHuIL-33)