Recombinant Murine Interleukin-7 (rMuIL-7)

英文全名

Recombinant Murine Interleukin-7 (rMuIL-7)

英文别名

LP-1, pre-B cell factor

蛋白结构

Squence of Recombinant Murine Interleukin-7

氨基酸序列

ECHIKDKEGK AYESVLMISI DELDKMTGTD SNCPNNEPNF FRKHVCDDTK EAAFLNRAAR KLKQFLKMNI SEEFNVHLLT VSQGTQTLVN CTSKEEKNVK EQKKNDACFL KRLLREIKTC WNKILKGSI

Accession

Q544C8

Gene ID

16196

产品英文概述

Interleukin-7 (IL-7) is encoded by the IL7 gene in mouse and secreted by stromal cells in the red marrow and thymus. The protein signals through the IL-7 receptor, which is a heterodimer consisting of IL-7 receptor alpha and IL-2 receptor gamma chain. IL-7 stimulates the differentiation of hematopoietic stem cells into lymphoid progenitor cells and it can stimulate proliferation of B cells, T cells and NK cells. Murine IL-7 has approximately 65 % and 88 % amino acid sequence identity with human and rat IL-7 and both proteins exhibit cross-species activity. IL-7 as an immunotherapy agent has been examined in many human clinical trials for various malignancies and during HIV infection.

来源

Escherichia coli.

分子量

Approximately 14.9 kDa, a single non-glycosylated polypeptide chain containing 129 amino acids.

生物活性

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine 2E8 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 106 IU/mg.

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, 2 % trehalose.

内毒素

Less than 1 EU/ug of rMuIL-7 as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

纯度

> 96 % by SDS-PAGE and HPLC analyses.
SDS-PAGE of Recombinant Murine Interleukin-7

包装规格

10ug/100ug/500ug

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品牌链接

Recombinant Murine Interleukin-7是ChemWhat品牌授权产品并通过沃尔森销售,下面是ChemWhat上的该产品链接Recombinant Murine Interleukin-7 (rMuIL-7)