Recombinant Murine Interleukin-2 (rMuIL-2)
T-cell growth factor, TCGF, Aldesleukin
APTSSSTSSS TAEAQQQQQQ QQQQQQHLEQ LLMDLQELLS RMENYRNLKL PRMLTFKFYL PKQATELKDL QCLEDELGPL RHVLDLTQSK SFQLEDAENF ISNIRVTVVK LKGSDNTFEC QFDDESATVV DFLRRWIAFC QSIISTSPQ
IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, gammadelta T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL2 consists of three subunits (55 kDa IL2Ralpha, 75 kDa IL2Rβ, 64 kDa common gamma chain gammac/IL2Rgamma) that are present on the cell surface in varying preformed complexes; Mature mouse IL2 shares 56 % and 73 % amino acid sequence identity with human and rat IL2 respectively. Mouse and human IL2 exhibit cross-species activity. Mouse IL2 has a specific N-terminal region that contains a poly glutamine (contain 12 glutamines) stretch.
Approximately 17.2 kDa, a single non-glycosylated polypeptide chain containing 149 amino acids.
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 106 IU/mg.
Sterile filtered white lyophilized (freeze-dried) powder.
Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
Less than 1 EU/ug of rMuIL-2 as determined by LAL method.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
> 95 % by SDS-PAGE and HPLC analyses.
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