Recombinant Human CLEC-1 Protein is expressed from HEK293 with hFc tag at the N-terminal.It contains Gln74-Asp280.
CLEC-1; CLEC1; CLEC1A; MGC34328
The protein has a predicted MW of 51.2 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Immobilized Human CLEC-1, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CLEC-1 Antibody, hFc Tag with the EC50 8.8ng/ml determined by ELISA. See testing image for detail.
> 95% as determined by Tris-Bis PAGE
Less than 1EU per ug by the LAL method.
Shipped at ambient temperature.
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.