Recombinant Cynomolgus/Rhesus macaque FcRn Protein is expressed from HEK293 with His tag at the C-terminal.It contains Ala24-Ser297 (FCGRT)&Ile21-Met119 (B2M).
FcRn alpha chain; FCGRT; FCRN; FCGRT&B2M
Ala24-Ser297(FCGRT) & Ile21-Met119(B2M)
The protein has a predicted MW of 31.5 kDa (FCGRT)&11.6 kDa (B2M). Due to glycosylation, the protein migrates to 35-38 kDa (FCGRT)&13-15 kDa (B2M) based on Tris-Bis PAGE result.
Immobilized Cynomolgus/Rhesus macaque FcRn, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 13.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.18 uM as determined in SPR assay (Biacore T200). See testing image for detail.
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Less than 1EU per ug by the LAL method.
Shipped at ambient temperature.
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.