Recombinant Biotinylated Human GARP&Latent TGF beta 1 Complex Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains His20-Leu628(GARP)&Leu30-Ser390(Latent TGF beta 1).
GARP&Latent TGF beta 1 Complex
LRRC32; GARP; LAP; TGF-beta-1; LRRC32&TGF-beta 1; LRRC32&TGFB1
Q14392(GARP)&P01137(Latent TGF beta 1)
His20-Leu628(GARP) & Leu30-Ser390(Latent TGF beta 1)
The protein has a predicted MW of 70.3 kDa (GARP)&41.4 kDa (Latent TGF beta 1). Due to glycosylation, the protein migrates to 73-78 kDa (GARP)&13 kDa&42-45 kDa (Latent TGF beta 1) based on Tris-Bis PAGE result.
Immobilized Anti-GARP&Latent TGF beta 1 Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GARP&Latent TGF Beta 1 Complex, His Tag with the EC50 of 44.0ng/ml determined by ELISA. See testing image for detail.
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Less than 1EU per ug by the LAL method.
Shipped at ambient temperature.
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.