Recombinant Viral Macrophage Inflammatory Protein-2 (rViMIP-2)
Viral Macrophage Inflammatory Protein II, vMIP-1B, MIP-II, vMIP-2
LGASWHRPDK CCLGYQKRPL PQVLLSSWYP TSQLCSKPGV IFLTKRGRQV CADKSKDWVK KLMQQLPVTA
Approximately 8.0 kDa, a single, non-glycosylated polypeptide chain containing 70 amino acids.
Fully biologically active when compared to standard. The specific activity is determined by the inhibitory effect on monocyte migration response to human MIP-1 alpha using a concentration range of 1.0ug-10.0ug/ml of viral MIP-2 will inhibit 25ng/ml of human MIP-1 alpha.
Sterile filtered white lyophilized (freeze-dried) powder.
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150mM NaCl.
Less than 1 EU/ug of rViMIP-2 as determined by LAL method.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
1. Liwang AC, Wang ZX, Sun Y, et al. 1999. Protein Sci, 8: 2270-80.2. Morris KV, Higgins J, Shen X, et al. 2003. Virus Res, 94: 103-12.3. Luttichau HR. 2008. Virol J, 5: 50.4. Shao W, Fernandez E, Sachpatzidis A, et al. 2001. Eur J Biochem, 268: 2948-59.
> 97 % by SDS-PAGE and HPLC analyses.
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