Recombinant Rat Keratinocyte Growth Factor-2/FGF-10 (rRtKGF-2/FGF-10)

英文全名

Recombinant Rat Keratinocyte Growth Factor-2/FGF-10 (rRtKGF-2/FGF-10)

英文别名

蛋白结构

Squence of Recombinant Rat Keratinocyte Growth Factor-2/FGF-10

氨基酸序列

QALGQDMVSP EATNSSSSSS SSSSSSSFSS PSSAGRHVRS YNHLQGDVRW RKLFSFTKYF LKIEKNGKVS GTKKENCPYS ILEITSVEIG VVAVKAINSN YYLAMNKKGK LYGSKEFNND CKLKERIEEN GYNTYASFNW QHNGRQMYVA LNGKGAPRRG QKTRRKNTSA HFLPMVVHS

Accession

P70492

Gene ID

25443

产品英文概述

Fibroblast growth factor 10 is a protein which belongs to the fibroblast growth factor (FGF) family and FGF family members are involved in a variety of biological processes such as embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. Like most other FGF family members, FGF-10 also has a heparin-binding domain and it plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. In addition, FGF-10 may play a role in wound healing and is required for normal branching morphogenesis. Recombinant rat FGF-10 contains a 179 amino acids and it shares 94 % and 100 % a.a. sequence identity with human and murine FGF-10.

来源

Escherichia coli.

分子量

Approximately 20.0 kDa, a single non-glycosylated polypeptide chain containing 179 amino acids.

生物活性

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using monkey 4MBr-5 cells is less than 120 ng/ml, corresponding to a specific activity of > 8.3 × 103 IU/mg.

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris, 500 mM NaCl, pH 7.4, 5 % trehalose.

内毒素

Less than 1 EU/ug of rRtKGF-2/FGF-10 as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

纯度

> 97 % by SDS-PAGE and HPLC analyses.
SDS-PAGE of Recombinant Rat Keratinocyte Growth Factor-2/FGF-10

包装规格

5ug/100ug/500ug

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品牌链接

Recombinant Rat Keratinocyte Growth Factor-2/FGF-10是ChemWhat品牌授权产品并通过沃尔森销售,下面是ChemWhat上的该产品链接Recombinant Rat Keratinocyte Growth Factor-2/FGF-10 (rRtKGF-2/FGF-10)