Recombinant Human Ubiquitin-conjugating Enzyme E2 K, His (rHuUBE2K,His)

英文全名

Recombinant Human Ubiquitin-conjugating Enzyme E2 K, His (rHuUBE2K,His)

英文别名

HIP-2, Ubiquitin Carrier Protein, Ubiquitin-protein Ligase

蛋白结构

Squence of Recombinant Human Ubiquitin-conjugating Enzyme E2 K, His

氨基酸序列

MHHHHHHAMA NIAVQRIKRE FKEVLKSEET SKNQIKVDLV DENFTELRGE IAGPPDTPYE GGRYQLEIKI PETYPFNPPK VRFITKIWHP NISSVTGAIC LDILKDQWAA AMTLRTVLLS LQALLAAAEP DDPQDAVVAN QYKQNPEMFK QTARLWAHVY AGAPVSSPEY TKKIENLCAM GFDRNAVIVA LSSKSWDVET ATELLLSN

Accession

P61086

Gene ID

3093

产品英文概述

Ubiquitin-conjugating enzyme E2 K belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2K gene in humans. The ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, take part in the second step in the ubiquitination reaction. In this reaction, E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue. The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region. The ubiquitination reaction can modify proteins and regulate protein degradation. The UBE2K has been shown to interact with Huntingtin and RNF2. Additionally, it has been implicated in the degradation of huntingtin and suppression of apoptosis.

来源

Escherichia coli.

分子量

Approximately 23.4 kDa, a single non-glycosylated polypeptide chain containing 200 amino acids (a.a.) of human UBE2K and 8 a.a. vector sequence including 6 × His tag at N-terminus.

生物活性

No data available

外观

Sterile Colorless liquid.

配方

A 0.2 um filtered concentrated solution in 50 mM HEPES, pH 8.0, with 100 mM NaCl, 10 % Glycerol, 5 % Trehalose, 1 mM DTT.

内毒素

Less than 1 EU/ug of rHuUBE2K, His as determined by LAL method.

溶解说明

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 6 months from date of receipt, -20 to -70 °C as supplied.- 3 months, -20 to -70 °C under sterile conditions after opening.

参考资料

纯度

> 95 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

SDS-PAGE of Recombinant Human Ubiquitin-conjugating Enzyme E2 K, His

包装规格

10ug/100ug/500ug

安全数据表下载 (SDS)

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品牌链接

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