Recombinant Human Interleukin-36 Receptor Antagonist Protein (rHuIL-36RA)

英文全名

Recombinant Human Interleukin-36 Receptor Antagonist Protein (rHuIL-36RA)

英文别名

IL-1RP3, IL-1HY1, IL-1 delta, IL-1F5, IL-1ra homolog 1, IL-1L1

蛋白结构

Squence of Recombinant Human Interleukin-36 Receptor Antagonist Protein

氨基酸序列

VLSGALCFRM KDSALKVLYL HNNQLLAGGL HAGKVIKGEE ISVVPNRWLD ASLSPVILGV QGGSQCLSCG VGQEPTLTLE PVNIMELYLG AKESKSFTFY RRDMGLTSSF ESAAYPGWFL CTVPEADQPV RLTQLPENGG WNAPITDFYF QQCD

Accession

Q9UBH0

Gene ID

26525

产品英文概述

Interleukin-36 receptor antagonist (IL-36RA) is a secreted protein which belongs to the interleukin 1 cytokine family (IL-1 family) and it is predominantly expressed in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. IL-36RA has been reported to antagonize the biological activity of IL-36alpha (IL-1F6), IL-36β (IL-1F8), and IL-36gamma (IL-1F9). Furthermore, it could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1). In addition, The receptor for IL-36RA has not been positively identified. Indirect evidence suggests it is IL-1Rrp2. Recombinant human IL-36 RA contains 154 amino acid residues and it shares 91 % a.a. sequence identity with murine IL-36RA.

来源

Escherichia coli.

分子量

Approximately 16.8 kDa, a single non-glycosylated polypeptide chain containing 154 amino acids.

生物活性

Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-36 beta induced IL-8 secretion by human preadipocytes is less than 1.0 ug/ml, corresponding to a specific activity of > 1000 IU/mg in the presence of 20 ng/ml of recombinant human IL-36 beta.

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.

内毒素

Less than 1 EU/ug of rHuIL-36RA as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

纯度

> 95 % by SDS-PAGE and HPLC analyses.
SDS-PAGE of Recombinant Human Interleukin-36 Receptor Antagonist Protein

包装规格

5ug/100ug/500ug

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品牌链接

Recombinant Human Interleukin-36 Receptor Antagonist Protein是ChemWhat品牌授权产品并通过沃尔森销售,下面是ChemWhat上的该产品链接Recombinant Human Interleukin-36 Receptor Antagonist Protein (rHuIL-36RA)