Recombinant Human Interleukin-36 alpha, 158a.a. (rHuIL-36alpha,158a.a.)

英文全名

Recombinant Human Interleukin-36 alpha, 158a.a. (rHuIL-36alpha,158a.a.)

英文别名

FIL1 epsilon, IL-1 epsilon, IL-1F6, IL-1H1

蛋白结构

Squence of Recombinant Human Interleukin-36 alpha, 158a.a.

氨基酸序列

MEKALKIDTP QQGSIQDINH RVWVLQDQTL IAVPRKDRMS PVTIALISCR HVETLEKDRG NPIYLGLNGL NLCLMCAKVG DQPTLQLKEK DIMDLYNQPE PVKSFLFYHS QSGRNSTFES VAFPGWFIAV SSEGGCPLIL TQELGKANTT DFGLTMLF

Accession

Q9UHA7

Gene ID

27179

产品英文概述

Interleukin-36 (IL-36) is a pro-inflammatory cytokine which plays an important role in the pathophysiology of several diseases. IL-36alpha, IL-36β, and IL-36gamma (formerly IL-1F6, IL-1F8, and IL-1F9) are IL-1 family members that signal through the IL-1 receptor family members IL-1Rrp2 (IL-1RL2) and IL-1RAcP. Studies showed IL-36alpha is mainly found in skin and lymphoid tissues, but also in fetal brain, trachea, stomach and intestine. Notably, IL-36 alpha is the only novel IL-1 family member expressed on T-cells. Recombinant human interleukin-36 alpha contains 158 amino acids residues which is a single non-glycosylated polypeptide and it is 30 % a.a. identical to IL-1ra, and 27 %, 31 %, 36 %, 46 %, 57% and 28 % a.a. identical to IL-1β, IL-36Ra/IL-1F5, IL-37/IL-1F7, IL-36β/IL-1F8, IL-36gamma/IL-1F9 and IL-1F10.

来源

Escherichia coli.

分子量

Approximately 17.7 kDa, a single non-glycosylated polypeptide chain containing 158 amino acids.

生物活性

Fully biologically active when compared to standard. The specific activity determined by its ability in a functional ELISA. Immobilized rHuIL-36α at 1 ug/mL can bind recombinant human IL-1 Rrp2 Fc Chimera with a range of 0.15-5 ug/mL.

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in 2 × PBS, pH 7.4.

内毒素

Less than 1 EU/ug of rHuIL-36α, 158a.a. as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

纯度

> 95 % by SDS-PAGE and HPLC analyses.
SDS-PAGE of Recombinant Human Interleukin-36 alpha, 158a.a.

包装规格

10ug/100ug/500ug

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技术参数表下载 (TDS)

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品牌链接

Recombinant Human Interleukin-36 alpha, 158a.a.是ChemWhat品牌授权产品并通过沃尔森销售,下面是ChemWhat上的该产品链接Recombinant Human Interleukin-36 alpha, 158a.a. (rHuIL-36alpha,158a.a.)