Recombinant Human Bone Morphogenetic Protein 2 (rHuBMP-2)

英文全名

Recombinant Human Bone Morphogenetic Protein 2 (rHuBMP-2)

英文别名

BMP-2A

蛋白结构

Squence of Recombinant Human Bone Morphogenetic Protein 2

氨基酸序列

MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR

Accession

P12643

Gene ID

650

产品英文概述

Bone Morphogenetic Protein 2 is one of the BMPs, some of which belong to the TGF-beta superfamily (BMP2-7). There are more than thirteen BMPs have been discovered nowadays and they are involved in inducing cartilage and bone formation. BMP-2 is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. It is a potent osteoinductive cytokine and plays its role in association with osteoconductive carriers such as collagen and synthetic hydroxyapatite. As it shown to stimulate the production of bone, the recombinant human BMP-2 is available for orthopaedic usage and dentisty. The functional form of BMP-2 is a disulflde-linked homodimer and about 26 kDa The precursor of BMP-2 is 259 a.a., containing the 23 a.a. N-terminal signal sequence which will be cleaved by a furin-type protease to form the mature peptide.

来源

Escherichia coli.

分子量

Approximately 26.0 kDa, a homodimeric protein consisting of two 115 amino acid non-glycosylated polypeptide chains.

生物活性

Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 200 ng/ml, corresponding to a specific activity of > 5.0 × 103 IU/mg.

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution containing 10 mM sodium citrate pH 3.5.

内毒素

Less than 1 EU/ug of rHuBMP-2 as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled H2O to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. Do not reconstitute in PBS or cell culture media.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

纯度

> 95 % by SDS-PAGE and HPLC analyses.
SDS-PAGE of Recombinant Human Bone Morphogenetic Protein 2

包装规格

10ug/100ug/500ug

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品牌链接

Recombinant Human Bone Morphogenetic Protein 2是ChemWhat品牌授权产品并通过沃尔森销售,下面是ChemWhat上的该产品链接Recombinant Human Bone Morphogenetic Protein 2 (rHuBMP-2)