Recombinant Human Beta-defensin 2 (rHuBD-2)

英文全名

Recombinant Human Beta-defensin 2 (rHuBD-2)

英文别名

Defensin beta2

蛋白结构

Squence of Recombinant Human Beta-defensin 2

氨基酸序列

GIGDPVTCLK SGAICHPVFC PRRYKQIGTC GLPGTKCCKK P

Accession

O15263

Gene ID

1673

产品英文概述

Defensins (alpha and beta) are cationic peptides with antimicrobial activity against Gram-negative and Gram-positive bacteria, fungi, and enveloped viruses. They are 2-6 kDa proteins and take important roles in innate immune system. On the basis of their size and pattern of disulfide bonding, mammalian defensins are classified into alpha, beta and theta categories. β-Defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Four human β-defensins have been identified and they are expressed on some leukocytes and at epithelial surfaces. Because β-defensins is cationic peptides, they can therefore interact with the membrane of invading microbes, which are negative due to lipopolysaccharides (LPS) and lipoteichoic acid (LTA) found in the cell membrane. Especially, they have higher affinity to the binding site compared to Ca2+ and Mg2+ ions. Furthermore, they can affect the stability of the membrane.

来源

Escherichia coli.

分子量

Approximately 4.3 kDa, a single non-glycosylated polypeptide chain containing 41 amino acids.

生物活性

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using immature human dendritic cells is in a concentration range of 10-100 ng/ml.

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl.

内毒素

Less than 1 EU/ug of rHuBD-2 as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

纯度

> 98 % by SDS-PAGE and HPLC analyses.
SDS-PAGE of Recombinant Human Beta-defensin 2

包装规格

5ug/100ug/500ug

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品牌链接

Recombinant Human Beta-defensin 2是ChemWhat品牌授权产品并通过沃尔森销售,下面是ChemWhat上的该产品链接Recombinant Human Beta-defensin 2 (rHuBD-2)