Recombinant Hirudin (rHirudin)

英文全名

Recombinant Hirudin (rHirudin)

英文别名

蛋白结构

Squence of Recombinant Hirudin

氨基酸序列

VVYTDCTESG QNLCLCEGSN VCGQGNKCIL GSDGEKNQCV TGEGTPGPQS HNDGDFEEPE EYL

Accession

P84590

Gene ID

产品英文概述

来源

Pichia Pastoris

分子量

Approximately 6.7 kDa, a single non-glycosylated polypeptide chain containing 63 amino acid residues.

生物活性

The biological activity is determined by chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific activity is no less than 14,000 ATU/mg protein.

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered solution of 20 mM PBS, pH 7.0, containing 2 % mannitol.

内毒素

Less than 1 EU/ug of rHirudin as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

1. Knapp A, Degenhardt T, Dodt J. 1992. J Biol Chem, 267: 24230-4.2. Pineo GFandHull RD. 1995. Curr Opin Hematol, 2: 380-5.3. Badimon L, Merino A, Badimon J, et al. 1991. Trends Cardiovasc Med, 1: 261-7.4. Nasir MA, Toth CA, Mittra RA. 1996. Am J Ophthalmol, 121: 554-60.5. Agnelli GandSonaglia F. 1997. Semin Thromb Hemost, 23: 143-8.

纯度

> 96 % by SDS-PAGE and HPLC analyses.
SDS-PAGE of Recombinant Hirudin

包装规格

10ug/100ug/500ug

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品牌链接

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