Recombinant Rat GARP&Latent TGF Beta 1 Complex Protein is expressed from HEK293 with His tag and Avi Tag at the C-terminal.It contains Ile18-Asn628(GARP)&Leu30-Arg278(Latent TGF Beta 1).
GARP&Latent TGF Beta 1 Complex
D3ZVD5(GARP)&P17246(Latent TGF Beta)
Ile18-Asn628(GARP) & Leu30-Arg278(Latent TGF Beta 1)
The protein has a predicted MW of 69.80 kDa (GARP)&41.28 kDa (Latent TGF beta 1). Due to glycosylation, the protein migrates to 70-80 kDa (GARP)&42-48 kDa&13 kDa (Latent TGF beta 1) based on Tris-Bis PAGE result.
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Less than 1EU per ug by the LAL method.
Shipped at ambient temperature.
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.