Recombinant Mouse IFN alpha/beta R1 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Glu27-Thr429.
IFN alpha/beta R1
AVP; IFN-alpha/beta R1; IFN-alpha-REC; IFNAR; IFNAR1; IFN-aR1; IFNBR; IFNbR1; IFN-R-1; CRF2-1; IFRC
The protein has a predicted MW of 46.9 kDa. Due to glycosylation, the protein migrates to 67-70 kDa based on Tris-Bis PAGE result.
The affinity constant of 3.61 nM as determined in SPR assay (Biacore T200). See testing image for detail.
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Less than 1EU per ug by the LAL method.
Shipped at ambient temperature.
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.