Recombinant Human&Mouse Chimeric NY-ESO-1 (HLA-A*02:01) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal, tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr206(Human HLA-A*02:01 α1&α2)&Asp140-Lys241(Mouse HLA-A*02:01 α3), Ile21-Met119 (B2M) and SLLMWITQC.
NY-ESO-1 (HLA-A*02:01) Tetramer
MHC; MY-ESO-1; CT6.1; LAGE-2; CTAG1; CTAG1B; ESO1CTAG; LAGE2A; NY-ESO-1
Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp140-Lys241(Mouse HLA-A*02:01 α3), Ile21-Met119(B2M)and SLLMWITQC
The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
Immobilized Human&Mouse Chimeric NY-ESO-1 (HLA-A*02:01) Tetramer, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-NY-ESO-1 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 17.3ng/ml determined by ELISA. See testing image for detail.
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Less than 1EU per ug by the LAL method.
Shipped with blue ice.
Supplied as 0.22um filtered solution in PBS (pH 7.4).
Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid repeated freeze-thaw cycles.