Recombinant Human&Mouse Chimeric LMP2 (HLA-A*02:01) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr206(Human HLA-A*02:01 α1&α2)&Asp207-Ala362(Mouse HLA-A*02:01 α3), Ile21-Met119(B2M) and CLGGLLTMV peptide.
LMP2 (HLA-A*02:01) Tetramer
MHC; RMF; LMP2; LMP-2; Macropain chain 7; Proteasome chain 7; PSMB9; RING12
Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Ala362(Mouse HLA-A*02:01 α3), Ile21-Met119(B2M)and CLGGLLTMV peptide
The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
Immobilized Human&Mouse Chimeric LMP2 (HLA-A*02:01) Tetramer, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LMP2 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 21.4ng/ml determined by ELISA. See testing image for detail.
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Less than 1EU per ug by the LAL method.
Shipped at ambient temperature.
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.