Recombinant Human TNFSF12 Protein is expressed from HEK293 with hFc tag at the C-terminal.It contains Ser43-His249.
The protein has a predicted MW of 49.62 kDa. Due to enzyme lysis and glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
Immobilized Human TNFSF12, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TNFSF12 Antibody, hFc Tag with the EC50 of 0.11ug/ml determined by ELISA (QC Test). The affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). See testing image for detail.
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Less than 1EU per ug by the LAL method.
Shipped at ambient temperature.
Lyophilized from 0.22um filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.