Recombinant Human NY-ESO-1 (HLA-A*02:01) Protein is expressed from E.coli with His tag and Avi tag at the C-terminal.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLMWITQC peptide.
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLMWITQC peptide
The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Tris-Bis PAGE result.
Immobilized Anti-NY-ESO-1 (HLA-A*02:01) Antibody at 5μg/ml (100μl/well) on the plate. Dose response curve for Human NY-ESO-1 (HLA-A*02:01) , His Tag with the EC50 of 7.2ng/ml determined by ELISA. See testing image for detail.
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Shipped with blue ice.
Supplied as 0.22μm filtered solution in 20mM Tris, 150mM NaCl (pH 8.0). Please dilute to the desired concentration according to the concentration of the solution shown on the product label.
Valid for 12 months from date of receipt when stored at -80°C.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.