Recombinant Human NKG2C&CD94 Protein is expressed from HEK293 with His tag and Avi tag at the N-terminal.It contains Glu98-Leu231(NKG2C)&Ser34-Ile179(CD94).
Glu98-Leu231(NKG2C) & Ser34-Ile179(CD94)
The protein has a predicted MW of 15.3kDa (NKG2C)&17.9kDa (CD94). Due to glycosylation, the protein migrates to 30-50 kDa based on Tris-Bis PAGE result.
The affinity constant of 1.65 uM as determined in SPR assay (Biacore T200). See testing image for detail.
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Less than 1EU per ug by the LAL method.
Shipped at ambient temperature.
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.