Human IL-27 Protein, Accession: Q14213

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  • 产品概述
  • 产品规格
  • 运输及存储
  • 品牌声明

    • 产品概述

    产品描述

    Recombinant Human IL-27 Protein is expressed from CHO with His tag at the C-terminal.It contains Arg21-Lys229(IL-27B)&Phe29-Pro243(IL-27A).

    Biotinylated

    产品规格

    靶点

    IL-27

    分子别名

    Accession

    Q14213

    种属

    Human

    蛋白序列

    Arg21-Lys229(IL-27B) & Phe29-Pro243(IL-27A)

    分子量

    The protein has a predicted MW of 50.6 kDa. Due to glycosylation, the protein migrates to 54-64 kDa based on Tris-Bis PAGE result.

    活性

    Immobilized Human IL-27 RA, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 22.7ng/ml determined by ELISA (QC Test). The affinity constant of 3.58 nM as determined in SPR assay (Biacore T200). See testing image for detail.

    产品标签

    C-His

    表达系统

    CHO

    纯度

    > 95% as determined by Tris-Bis PAGE

    内毒素

    Less than 1EU per ug by the LAL method.

    运输及存储

    产品外观

    Lyophilized

    运输条件

    Shipped at ambient temperature.

    制剂

    Lyophilized from 0.22um filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

    复溶

    Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

    存储和稳定性

    Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

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