Human HGF Protein, Accession: P14210

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  • 产品概述
  • 产品规格
  • 运输及存储
  • 品牌声明

    • 产品概述

    产品描述

    Recombinant Human HGF Protein is expressed from Expi293 with hFc tag at the C-terminal.It contains Gln32-Ser728.

    Biotinylated

    产品规格

    靶点

    HGF

    分子别名

    Hepatopoietin-A; SF; HPTA; HGF; DFNB39; F-TCF; F-TCFB; HGFB

    Accession

    P14210

    种属

    Human

    蛋白序列

    Gln32-Ser728

    分子量

    The HGF single chain can be processed into the active form of disulfide-linked heterodimer of α and β chain.The protein has a predicted MW of 79.6 kDa (after cleavage of the signal peptide). Due to glycosylation, the protein migrates to 60-65 kDa corresponding to α chain and β chain based on Bis-Tris PAGE result.

    活性

    Immobilized Human HGF R, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human HGF, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA. See testing image for detail.

    产品标签

    C-hFc

    表达系统

    Expi293

    纯度

    > 95% as determined by Tris-Bis PAGE

    内毒素

    Less than 1EU per μg by the LAL method.

    运输及存储

    产品外观

    Lyophilized

    运输条件

    Shipped at ambient temperature.

    制剂

    Lyophilized from 0.22μm filtered solution in 20mM Tris, 150mM NaCl, 300mM Arginine (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.

    复溶

    Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

    存储和稳定性

    Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

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