Recombinant Cynomolgus Siglec-10 Protein is expressed from HEK293 with His tag at the C-terminal.It contains Thr17-Asn552.
The protein has a predicted MW of 59.90 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Immobilized Cynomolgus Siglec-10, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 42.0ng/ml determined by ELISA. See testing image for detail.
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Less than 1EU per ug by the LAL method.
Shipped with blue ice.
Supplied as 0.22um filtered solution in 25mM MES, 150mM NaCl, 0.5M Arginine (pH 5.0).
Valid for 12 months from date of receipt when stored at -80°C.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid repeated freeze-thaw cycles.