Recombinant Cynomolgus CD44 Protein is expressed from Expi293 with His tag at the C-terminal.It contains Gln21-Pro220.
The protein has a predicted MW of 23.03 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Shipped at ambient temperature.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.