Recombinant Canine GDF15 Protein is expressed from E.coli with His tag at the N-terminal.It contains Ala197-Val307.
The protein has a predicted MW of 13.12 kDa. The protein migrates to 15-20 kDa under reduced (R) condition and 23-26 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
Immobilized Canine GDF15, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-GDF15 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA (QC Test). See testing image for detail.
> 95% as determined by Tris-Bis PAGE
Less than 1EU per ug by the LAL method.
Shipped at ambient temperature.
Lyophilized from 0.22um filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9).
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.