Recombinant Biotinylated Mouse CX3CL1/Fractalkine Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Gln25-Thr336.
Fractalkine; Neurotactin; Cx3cl1; Cx3c; Fkn; ABCD-3; C3Xkine; CXC3; CXC3C; NTN; NTT; SCYD1
The protein has a predicted MW of 36 kDa. Due to glycosylation, the protein migrates to 70-83 kDa based on Tris-Bis PAGE result.
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Less than 1EU per ug by the LAL method.
Shipped at ambient temperature.
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.