Recombinant Biotinylated Human TNFR2/CD120b/TNFRSF1B Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-terminal.It contains Leu23-Asp257.
The protein has a predicted MW of 26.2 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Tris-Bis PAGE result.
Immobilized Anti-TNFR2 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TNFR2, His Tag with the EC50 of 33.8ng/ml determined by ELISA. See testing image for detail.
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Less than 1EU per ug by the LAL method.
Shipped with blue ice.
Supplied as 0.22um filtered solution in PBS (pH 7.4).
Valid for 12 months from date of receipt when stored at -80°C.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid repeated freeze-thaw cycles.