Recombinant Biotinylated Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Phe42-Tyr995(ITGA5) acidic tail&Gln21-Asp728(ITGB1) basic tail.
Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer
Phe42-Tyr995(ITGA5)acidic tail & Gln21-Asp728(ITGB1)basic tail
The protein has a predicted MW of 111.8 kDa (ITGA5)&83.2 kDa (ITGB1). Due to glycosylation, the protein migrates to 110-140 kDa based on Tris-Bis PAGE result.
Immobilized 0.5ug Anti-ITGA5&ITGB1 Antibody, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human ITGA5&ITGB1, His Tag with the EC50 of 55.3ng/ml determined by ELISA. See testing image for detail.
> 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Less than 1EU per ug by the LAL method.
Shipped at ambient temperature.
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.