Recombinant Biotinylated Human FAP Protein is expressed from HEK293 with His tag and Avi tag at the N-terminal.It contains Leu26-Asp760.
FAP; FAPalpha; SIMP; Seprase; APCE; DPPIV; DPPIVA; FAPA
The protein has a predicted MW of 87.9 kDa. Due to glycosylation, the protein migrates to 90-100 kDa based on Tris-Bis PAGE result.
Immobilized Anti-FAP Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FAP, His Tag with the EC50 of 55.6ng/ml determined by ELISA. See testing image for detail.
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Less than 1EU per ug by the LAL method.
Shipped at ambient temperature.
Lyophilized from 0.22um filtered solution in 20mM Tirs, 150mM NaCl, 200mM L-Arginine (pH 8.2). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.