Recombinant Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein is expressed from HEK293 with hFc tag and Avi tag at the C-terminal.It contains Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D).
CD3E&CD3D/CD3 epsilon&CD3 delta
CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon
Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D)
The protein has a predicted MW of 40 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation,the protein migrates to 48-60 kDa based on Tris-Bis PAGE result.
Immobilized Biotinylated Human CD3E&CD3D, hFc Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for OKT3, mFc Tag with the EC50 of 42.1ng/ml determined by ELISA. See testing image for detail.
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Less than 1EU per ug by the LAL method.
Shipped at ambient temperature.
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.