Recombinant Biotinylated Human CA125/MUC16 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Gly12660-Met12923.
CA125; CA-125; CA125MUC-16; FLJ14303; MUC16
The protein has a predicted MW of 31.3 kDa. Due to glycosylation, the protein migrates to 52-80 kDa based on Tris-Bis PAGE result.
Immobilized Human MSLN, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CA125, His Tag with the EC50 of 0.2ug/ml determined by ELISA. See testing image for detail.
> 95% as determined by Tris-Bis PAGE
Less than 1EU per ug by the LAL method.
Shipped at ambient temperature.
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.