Recombinant Biotinylated Human APOE3/Apolipoprotein E Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the N-terminal.It contains Lys19-His317.
Apolipoprotein E; Apo-E; APOE; apolipo E
The protein has a predicted MW of 35.3 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.
The affinity constant of 1.41 uM as determined in SPR assay (Biacore T200). See testing image for detail.
> 95% as determined by Tris-Bis PAGE
Less than 1EU per ug by the LAL method.
Shipped at ambient temperature.
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.