Recombinant Biotinylated Human AFP (HLA-A*02:01) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and FMNKFIYEI peptide.
Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and FMNKFIYEI peptide
The protein has a predicted MW of 50.6 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Tris-Bis PAGE result.
Immobilized Anti-AFP (HLA-A*02:01) Antibody, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human AFP (HLA-A*02:01) , His Tag with the EC50 of 0.31ug/ml determined by ELISA. See testing image for detail.
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Less than 1EU per ug by the LAL method.
Shipped at ambient temperature.
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.